Plasmid name :
pcDNA3.1(+)-3*HA-ALKBH5 Gene/insert name :
ALKBH5 Alt name :
ABH5; OFOXD; OFOXD1 Insert size :
1185bp Species :
Homo sapiens (human) Gene ID :
54890 Accessions :
NM_017758.4 Vector Backbone :
pcDNA3.1(+) Tag :
3*HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top 10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens alkB homolog 5, RNA demethylase(one point mutation: C1017T not causing the amino acid variation) with N terminal 3xHA tag Selectable markers :
Neomycin
pcDNA3.1(+)-3*HA-ALKBH5 Gene/insert name :
ALKBH5 Alt name :
ABH5; OFOXD; OFOXD1 Insert size :
1185bp Species :
Homo sapiens (human) Gene ID :
54890 Accessions :
NM_017758.4 Vector Backbone :
pcDNA3.1(+) Tag :
3*HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top 10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens alkB homolog 5, RNA demethylase(one point mutation: C1017T not causing the amino acid variation) with N terminal 3xHA tag Selectable markers :
Neomycin
Blocking peptide available as PPL01983-2aP