Plasmid name :
pcDNA3.1(+)-3*HA-WRN Gene/insert name :
WRN Alt name :
RECQ3; RECQL2; RECQL3 Insert size :
4299bp Species :
Homo sapiens (human) Gene ID :
7486 Accessions :
NM_000553.6 Vector Backbone :
pcDNA3.1(+) Tag :
3*HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top 10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens WRN RecQ like helicase(three point mutations G252A/C513T/C4083T not causing the amino acid variation) with N terminal 3xHA tag Selectable markers :
Neomycin

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Blocking peptide available as PPL01984-2aP