Plasmid name :
p3*Flag-TP53(R249S) Gene/insert name :
TP53(R249S) Alt name :
P53; BCC7; LFS1; BMFS5; TRP53 Insert size :
1182bp Species :
Homo sapiens (human) Gene ID :
7157 Accessions :
AF307851 Vector Backbone :
p3xFLAG-Myc-CMV-24 Tag :
3*Flag(N-term) Backbone manufacturer :
Sigma Vector type :
Mammalian Expression Backbone size w/o insert :
4800bp Cloning site 5' :
Sal I Site destroyed during cloning :
Yes Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens tumor protein p53(one points mutation R249S) with N terminal 3xFlag tag Selectable markers :
None
p3*Flag-TP53(R249S) Gene/insert name :
TP53(R249S) Alt name :
P53; BCC7; LFS1; BMFS5; TRP53 Insert size :
1182bp Species :
Homo sapiens (human) Gene ID :
7157 Accessions :
AF307851 Vector Backbone :
p3xFLAG-Myc-CMV-24 Tag :
3*Flag(N-term) Backbone manufacturer :
Sigma Vector type :
Mammalian Expression Backbone size w/o insert :
4800bp Cloning site 5' :
Sal I Site destroyed during cloning :
Yes Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens tumor protein p53(one points mutation R249S) with N terminal 3xFlag tag Selectable markers :
None
Blocking peptide available as PPL00015-2jP