Plasmid name :
pcDNA3.1(+)-HA-KDM3A-3*Flag Gene/insert name :
KDM3A Alt name :
TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A Insert size :
3966bp Species :
Homo sapiens (human) Gene ID :
55818 Accessions :
NM_018433 Vector Backbone :
pcDNA3.1(+) Tag :
HA(N-term);3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens lysine demethylase 3A(one point mutation G918A not causing the amino acid variation) with N terminal HA tag and C terminal 3*Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-HA-KDM3A-3*Flag Gene/insert name :
KDM3A Alt name :
TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A Insert size :
3966bp Species :
Homo sapiens (human) Gene ID :
55818 Accessions :
NM_018433 Vector Backbone :
pcDNA3.1(+) Tag :
HA(N-term);3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens lysine demethylase 3A(one point mutation G918A not causing the amino acid variation) with N terminal HA tag and C terminal 3*Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL01950-2aP