Plasmid name :
pIRES2-EGFP-3*Flag-AANAT Gene/insert name :
AANAT Alt name :
N/A Insert size :
594bp Species :
Physeter catodon (sperm whale) Gene ID :
103011665 Accessions :
XM_007185968 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Balaenoptera acutorostrata scammoni aralkylamine N-acetyltransferase (AANAT) with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-AANAT Gene/insert name :
AANAT Alt name :
N/A Insert size :
594bp Species :
Physeter catodon (sperm whale) Gene ID :
103011665 Accessions :
XM_007185968 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Balaenoptera acutorostrata scammoni aralkylamine N-acetyltransferase (AANAT) with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL80076-2aP