Plasmid name :
pcDNA3-HA-Akt(DN) Gene/insert name :
Akt(DN) Alt name :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA Insert size :
1500bp Species :
Homo sapiens (human) Gene ID :
N/A Accessions :
N/A Vector Backbone :
pcDNA3 Tag :
HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5400bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(dominant negative mutation) with N terminal HA tag Selectable markers :
Neomycin
pcDNA3-HA-Akt(DN) Gene/insert name :
Akt(DN) Alt name :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA Insert size :
1500bp Species :
Homo sapiens (human) Gene ID :
N/A Accessions :
N/A Vector Backbone :
pcDNA3 Tag :
HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5400bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(dominant negative mutation) with N terminal HA tag Selectable markers :
Neomycin
Blocking peptide available as PPL00033-2bP