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pcDNA3.1(+)-Flag-AKT1(T308D/S473D) PPL00033-2f
Plasmid namepcDNA3.1(+)-Flag-AKT1(T308D/S473D)
Catalog No.PPL00033-2f
Gene/insert name
Alt nameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA
Insert size1443bp
SpeciesHomo sapiens (human)
Gene ID207
AccessionsNM_005163
Vector BackbonepcDNA3.1(+)
TagFlag(N-term)
Backbone manufacturerInvitrogen
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Plasmid name :
pcDNA3.1(+)-Flag-AKT1(T308D/S473D)
Gene/insert name :
AKT1(T308D/S473D)
Alt name :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA
Insert size :
1443bp
Species :
Homo sapiens (human)
Gene ID :
207
Accessions :
NM_005163
Vector Backbone :
pcDNA3.1(+)
Tag :
Flag(N-term)
Backbone manufacturer :
Invitrogen
Vector type :
Mammalian Expression
Backbone size w/o insert :
5428bp
Cloning site 5' :
Kpn I
Site destroyed during cloning :
NO
Cloning site 3' :
EcoR I
Site destroyed during cloning :
NO
Bacterial resistance(s) :
Ampicillin
Growth strain(s) :
Top10
Growth temperature :
37°C
High or low copy :
High Copy
Plasmid description :
Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(two points mutation T308D/S473D) with N terminal Flag tag
Selectable markers :
Neomycin
Blocking peptide available as PPL00033-2fP
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