Plasmid name :
pcDNA3.1(+)-Flag-AKT1(T308D/S473D) Gene/insert name :
AKT1(T308D/S473D) Alt name :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA Insert size :
1443bp Species :
Homo sapiens (human) Gene ID :
207 Accessions :
NM_005163 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Kpn I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(two points mutation T308D/S473D) with N terminal Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-Flag-AKT1(T308D/S473D) Gene/insert name :
AKT1(T308D/S473D) Alt name :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA Insert size :
1443bp Species :
Homo sapiens (human) Gene ID :
207 Accessions :
NM_005163 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Kpn I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(two points mutation T308D/S473D) with N terminal Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL00033-2fP