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pIRES2-EGFP-3*Flag-Alox12 PPL50129-2a
Plasmid namepIRES2-EGFP-3*Flag-Alox12
Catalog No.PPL50129-2a
Gene/insert name
Alt name9930022G08Rik; Alox12p; P-12LO
Insert size1992bp
SpeciesMus musculus (house mouse)
Gene ID11684
AccessionsNM_007440
Vector BackbonepIRES2-EGFP
Tag3*Flag(N-term);EGFP(C-term)
Backbone manufacturerClontech
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Plasmid name :
pIRES2-EGFP-3*Flag-Alox12
Gene/insert name :
Alox12
Alt name :
9930022G08Rik; Alox12p; P-12LO
Insert size :
1992bp
Species :
Mus musculus (house mouse)
Gene ID :
11684
Accessions :
NM_007440
Vector Backbone :
pIRES2-EGFP
Tag :
3*Flag(N-term);EGFP(C-term)
Backbone manufacturer :
Clontech
Vector type :
Mammalian Expression
Backbone size w/o insert :
5300bp
Cloning site 5' :
Xho I
Site destroyed during cloning :
NO
Cloning site 3' :
Sal I
Site destroyed during cloning :
NO
Bacterial resistance(s) :
Kanamycin
Growth strain(s) :
Top10
Growth temperature :
37°C
High or low copy :
High Copy
Plasmid description :
Mammalian Expression vector of Mus musculus arachidonate 12-lipoxygenase with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus
Selectable markers :
Neomycin
Blocking peptide available as PPL50129-2aP
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