Plasmid name :
pcDNA3-HA-ASK1(T1308I) Gene/insert name :
ASK1(T1308I) Alt name :
MAP3K5; MEKK5; MAPKKK5 Insert size :
4125bp Species :
Homo sapiens (human) Gene ID :
4217 Accessions :
NM_005923.3 Vector Backbone :
pcDNA3 Tag :
HA(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5400bp Cloning site 5' :
N/A Site destroyed during cloning :
N/A Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens mitogen-activated protein kinase kinase kinase 5(one pint mutation T1308I and four points mutation C159T/T555C/A594C/A1557G not causing the amino acid variation) with C terminal HA tag Selectable markers :
Neomycin

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Blocking peptide available as PPL00591-2aP