Plasmid name :
pLenti-CMV-GFP-Puro-Beclin1(E186G) Gene/insert name :
Beclin1(E186G) Alt name :
BECN1,ATG6,VPS30 Insert size :
1353bp Species :
Homo sapiens (human) Gene ID :
8678 Accessions :
NM_003766.3 Vector Backbone :
pLenti-CMV-GFP-Puro Tag :
CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Homo sapiens beclin 1(one point mutation E186G) with unfused CopGFP protein at the C-terminus.Identical with the NCBI sequence except for one point mutation: 837 T/C not causing the amino acid variation. Selectable markers :
Puromycin
pLenti-CMV-GFP-Puro-Beclin1(E186G) Gene/insert name :
Beclin1(E186G) Alt name :
BECN1,ATG6,VPS30 Insert size :
1353bp Species :
Homo sapiens (human) Gene ID :
8678 Accessions :
NM_003766.3 Vector Backbone :
pLenti-CMV-GFP-Puro Tag :
CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Homo sapiens beclin 1(one point mutation E186G) with unfused CopGFP protein at the C-terminus.Identical with the NCBI sequence except for one point mutation: 837 T/C not causing the amino acid variation. Selectable markers :
Puromycin
Blocking peptide available as PPL00043-4aP