Plasmid name :
pPLK/GFP+Puro-BMI-1 shRNA-1 Gene/insert name :
BMI-1 shRNA-1 Alt name :
BMI1; PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1 Insert size :
57bp Species :
Homo sapiens (human) Gene ID :
648 Accessions :
Vector Backbone :
pPLK/GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian shRNA Knockdown vector of Homo sapiens BMI1 proto-oncogene, polycomb ring finger Selectable markers :
Puromycin
pPLK/GFP+Puro-BMI-1 shRNA-1 Gene/insert name :
BMI-1 shRNA-1 Alt name :
BMI1; PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1 Insert size :
57bp Species :
Homo sapiens (human) Gene ID :
648 Accessions :
Vector Backbone :
pPLK/GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian shRNA Knockdown vector of Homo sapiens BMI1 proto-oncogene, polycomb ring finger Selectable markers :
Puromycin
Blocking peptide available as PPL00731-3aP