Plasmid name :
pIRES2-EGFP-Flag-peptide-CD63 Gene/insert name :
CD63 Alt name :
MLA1; ME491; LAMP-3; OMA81H; TSPAN30 Insert size :
717bp Species :
Homo sapiens (human) Gene ID :
967 Accessions :
BC002349 Vector Backbone :
pIRES2-EGFP Tag :
Flag(N-term);CKGGRAKDC peptide(N-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens CD63 molecule fused with N terminal Flag tag and the CKGGRAKDC peptide and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-Flag-peptide-CD63 Gene/insert name :
CD63 Alt name :
MLA1; ME491; LAMP-3; OMA81H; TSPAN30 Insert size :
717bp Species :
Homo sapiens (human) Gene ID :
967 Accessions :
BC002349 Vector Backbone :
pIRES2-EGFP Tag :
Flag(N-term);CKGGRAKDC peptide(N-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens CD63 molecule fused with N terminal Flag tag and the CKGGRAKDC peptide and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL00300-2aP