Plasmid name :
pIRES2-EGFP-3*Flag-cCDKN1B Gene/insert name :
CDKN1B Alt name :
N/A Insert size :
597bp Species :
Bos taurus (cattle) Gene ID :
512613 Accessions :
NM_001100346.1 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Bos taurus cyclin dependent kinase inhibitor 1B with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-cCDKN1B Gene/insert name :
CDKN1B Alt name :
N/A Insert size :
597bp Species :
Bos taurus (cattle) Gene ID :
512613 Accessions :
NM_001100346.1 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Bos taurus cyclin dependent kinase inhibitor 1B with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL80093-2aP