Plasmid name :
pIRES2-EGFP-3*Flag-mCdkn1b Gene/insert name :
Cdkn1b Alt name :
p27; Kip1; p27Kip1; AA408329; AI843786 Insert size :
594bp Species :
Mus musculus (house mouse) Gene ID :
12576 Accessions :
NM_009875.4 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus cyclin-dependent kinase inhibitor 1B with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-mCdkn1b Gene/insert name :
Cdkn1b Alt name :
p27; Kip1; p27Kip1; AA408329; AI843786 Insert size :
594bp Species :
Mus musculus (house mouse) Gene ID :
12576 Accessions :
NM_009875.4 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus cyclin-dependent kinase inhibitor 1B with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL50156-2a P