Plasmid name :
pIRES2-EGFP-3*Flag-Cflar Gene/insert name :
Cflar Alt name :
2310024N18Rik; A430105C05Rik; c-Flip; Cash;Casper; CLARP; FLAME; FLAME-1; Flip; Gm9845; I-FLICE;MRIT Insert size :
1446bp Species :
Mus musculus (house mouse) Gene ID :
12633 Accessions :
NM_001289704 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus CASP8 and FADD-like apoptosis regulator with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-Cflar Gene/insert name :
Cflar Alt name :
2310024N18Rik; A430105C05Rik; c-Flip; Cash;Casper; CLARP; FLAME; FLAME-1; Flip; Gm9845; I-FLICE;MRIT Insert size :
1446bp Species :
Mus musculus (house mouse) Gene ID :
12633 Accessions :
NM_001289704 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus CASP8 and FADD-like apoptosis regulator with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL50127-2aP