Plasmid name :
pPLK/GFP+Puro-EGFR shRNA(3+1) Gene/insert name :
EGFR shRNA Alt name :
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2 Insert size :
~60bp Species :
Homo sapiens (human) Gene ID :
1956 Accessions :
Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pPLK/GFP+Puro-EGFR shRNA(3+1) Gene/insert name :
EGFR shRNA Alt name :
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2 Insert size :
~60bp Species :
Homo sapiens (human) Gene ID :
1956 Accessions :
Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL00063-3P