Plasmid name :
pLKO-Puro-hFAM129B shRNA(3+1) Gene/insert name :
FAM129B shRNA Alt name :
OC58; MEG-3; C9orf88; MINERVA; bA356B19.6 Insert size :
58bp Species :
Homo sapiens (human) Gene ID :
64855 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference, Inducible Backbone size w/o insert :
10633bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pLKO-Puro-hFAM129B shRNA(3+1) Gene/insert name :
FAM129B shRNA Alt name :
OC58; MEG-3; C9orf88; MINERVA; bA356B19.6 Insert size :
58bp Species :
Homo sapiens (human) Gene ID :
64855 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference, Inducible Backbone size w/o insert :
10633bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL01717-3P