Plasmid name :
pLVX-AcGFP1-N1-FTO(H231A/D233A) Gene/insert name :
FTO(H231A/D233A) Alt name :
GDFD; ALKBH9; BMIQ14 Insert size :
1518bp Species :
Homo sapiens (human) Gene ID :
79068 Accessions :
NM_001080432.2 Vector Backbone :
pLVX-AcGFP1-N1 Tag :
AcGFP1(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8834bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
Sma I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression of Homo sapiens FTO, alpha-ketoglutarate dependent dioxygenase(two point mutations H231A/D233A) with the AcGFP1 protein at the C-terminus Selectable markers :
Puromycin

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Blocking peptide available as PPL01645-4bP