Plasmid name :
PX458-PDCD1 Gene/insert name :
gRNA against PDCD1 Alt name :
CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1;SLEB2 Insert size :
20bp Species :
Homo sapiens (human) Gene ID :
5133 Accessions :
NM_005018.2 Vector Backbone :
PX458 Tag :
GFP(C-term) Backbone manufacturer :
Clontech Vector type :
CRISPR Backbone size w/o insert :
9289bp Cloning site 5' :
Bbs I Site destroyed during cloning :
Yes Cloning site 3' :
Bbs I Site destroyed during cloning :
Yes Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
CRISPR vector encoding gRNA for 3' target of Homo sapiens programmed cell death 1 Selectable markers :
None
PX458-PDCD1 Gene/insert name :
gRNA against PDCD1 Alt name :
CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1;SLEB2 Insert size :
20bp Species :
Homo sapiens (human) Gene ID :
5133 Accessions :
NM_005018.2 Vector Backbone :
PX458 Tag :
GFP(C-term) Backbone manufacturer :
Clontech Vector type :
CRISPR Backbone size w/o insert :
9289bp Cloning site 5' :
Bbs I Site destroyed during cloning :
Yes Cloning site 3' :
Bbs I Site destroyed during cloning :
Yes Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
CRISPR vector encoding gRNA for 3' target of Homo sapiens programmed cell death 1 Selectable markers :
None
Blocking peptide available as PPL00464-5aP