Plasmid name :
pcDNA3.1(+)-HA-GSDMD Gene/insert name :
GSDMD Alt name :
DF5L; DFNA5L; FKSG10; GSDMDC1 Insert size :
1455bp Species :
Homo sapiens (human) Gene ID :
79792 Accessions :
NM_024736.7 Vector Backbone :
pcDNA3.1(+) Tag :
HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
Xba I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens gasdermin D(two point mutations: G327T/G1023A not causing the amino acid variation) with N terminal HA tag Selectable markers :
Neomycin
pcDNA3.1(+)-HA-GSDMD Gene/insert name :
GSDMD Alt name :
DF5L; DFNA5L; FKSG10; GSDMDC1 Insert size :
1455bp Species :
Homo sapiens (human) Gene ID :
79792 Accessions :
NM_024736.7 Vector Backbone :
pcDNA3.1(+) Tag :
HA(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
Xba I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens gasdermin D(two point mutations: G327T/G1023A not causing the amino acid variation) with N terminal HA tag Selectable markers :
Neomycin
Blocking peptide available as PPL00857-2dP