Plasmid name :
pcDNA3.1(-)-Hif1a Gene/insert name :
Hif1a Alt name :
MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha Insert size :
2511bp Species :
Mus musculus (house mouse) Gene ID :
15251 Accessions :
NM_010431.2 Vector Backbone :
pcDNA3.1(-) Tag :
None Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5427bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus hypoxia inducible factor 1, alpha subunit(Identical with the NCBI sequence except for two point mutation: A480G/T576C not causing the amino acid variation) Selectable markers :
Neomycin
pcDNA3.1(-)-Hif1a Gene/insert name :
Hif1a Alt name :
MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha Insert size :
2511bp Species :
Mus musculus (house mouse) Gene ID :
15251 Accessions :
NM_010431.2 Vector Backbone :
pcDNA3.1(-) Tag :
None Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5427bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus hypoxia inducible factor 1, alpha subunit(Identical with the NCBI sequence except for two point mutation: A480G/T576C not causing the amino acid variation) Selectable markers :
Neomycin
Blocking peptide available as PPL50044-2aP