Plasmid name :
pcDNA3.1-3*Flag-HIF1A(D349N) Gene/insert name :
HIF1A(D349N) Alt name :
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA Insert size :
2481bp Species :
Homo sapiens (human) Gene ID :
3091 Accessions :
NM_001530.3 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens hypoxia inducible factor 1 alpha subunit(one point mutation D349N) with N terminal 3xFlag tag Selectable markers :
Neomycin
pcDNA3.1-3*Flag-HIF1A(D349N) Gene/insert name :
HIF1A(D349N) Alt name :
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA Insert size :
2481bp Species :
Homo sapiens (human) Gene ID :
3091 Accessions :
NM_001530.3 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens hypoxia inducible factor 1 alpha subunit(one point mutation D349N) with N terminal 3xFlag tag Selectable markers :
Neomycin
Blocking peptide available as PPL00599-2dP