Plasmid name :
pET14b-mHMGB1-EGFP Gene/insert name :
HMGB1 Alt name :
HMG-1; Hmg1; p30; SBP-1 Insert size :
648bp Species :
Mus musculus (house mouse) Gene ID :
15289 Accessions :
NM_001313894.1 Vector Backbone :
pET-14b-EGFP Tag :
His(N-term);EGFP(C-term) Backbone manufacturer :
N/A Vector type :
E. coli Expression Backbone size w/o insert :
6030bp Cloning site 5' :
Nde I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
TOP10 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
E. coli Expression vector of Mus musculus high mobility group box 1 with N terminal 6xHis tag and the fused EGFP protein at the C-terminus Selectable markers :
None
pET14b-mHMGB1-EGFP Gene/insert name :
HMGB1 Alt name :
HMG-1; Hmg1; p30; SBP-1 Insert size :
648bp Species :
Mus musculus (house mouse) Gene ID :
15289 Accessions :
NM_001313894.1 Vector Backbone :
pET-14b-EGFP Tag :
His(N-term);EGFP(C-term) Backbone manufacturer :
N/A Vector type :
E. coli Expression Backbone size w/o insert :
6030bp Cloning site 5' :
Nde I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
TOP10 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
E. coli Expression vector of Mus musculus high mobility group box 1 with N terminal 6xHis tag and the fused EGFP protein at the C-terminus Selectable markers :
None
Blocking peptide available as PPL50051-1aP