Plasmid name :
pcDNA3.1-Flag-hsp70 Gene/insert name :
hsp70 Alt name :
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103 Insert size :
1926bp Species :
Homo sapiens (human) Gene ID :
3303 Accessions :
NM_005345.5 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens heat shock protein family A (Hsp70)(four point mutations T222C/C678T/G1710T/C1905T not causing the amino acid variation) member 1A with N terminal Flag tag Selectable markers :
Neomycin
pcDNA3.1-Flag-hsp70 Gene/insert name :
hsp70 Alt name :
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103 Insert size :
1926bp Species :
Homo sapiens (human) Gene ID :
3303 Accessions :
NM_005345.5 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens heat shock protein family A (Hsp70)(four point mutations T222C/C678T/G1710T/C1905T not causing the amino acid variation) member 1A with N terminal Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL00012-2aP