Plasmid name :
pCD513B-1-MCM2 Gene/insert name :
MCM2 Alt name :
BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN Insert size :
2715bp Species :
Homo sapiens (human) Gene ID :
4171 Accessions :
BC007938.2 Vector Backbone :
pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro Tag :
Flag(N-term);HA(C-term);CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
Xba I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Homo sapiens minichromosome maintenance complex component 2 with N terminal Flag tag and C terminal HA tag and the unfused CopGFP protein at the C-terminus Selectable markers :
Puromycin
pCD513B-1-MCM2 Gene/insert name :
MCM2 Alt name :
BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN Insert size :
2715bp Species :
Homo sapiens (human) Gene ID :
4171 Accessions :
BC007938.2 Vector Backbone :
pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro Tag :
Flag(N-term);HA(C-term);CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
Xba I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Homo sapiens minichromosome maintenance complex component 2 with N terminal Flag tag and C terminal HA tag and the unfused CopGFP protein at the C-terminus Selectable markers :
Puromycin
Blocking peptide available as PPL00792-2aP