Plasmid name :
pcDNA3.1(+)-mDnmt1-T2A-EGFP Gene/insert name :
mDnmt1 Alt name :
Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2 Insert size :
4860bp Species :
Mus musculus (house mouse) Gene ID :
13433 Accessions :
NM_001199431.1 Vector Backbone :
pcDNA3.1(+) Tag :
T2A-EGFP(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus DNA methyltransferase (cytosine-5) 1, fused with the EGFP protein at the C-terminus Selectable markers :
Neomycin
pcDNA3.1(+)-mDnmt1-T2A-EGFP Gene/insert name :
mDnmt1 Alt name :
Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2 Insert size :
4860bp Species :
Mus musculus (house mouse) Gene ID :
13433 Accessions :
NM_001199431.1 Vector Backbone :
pcDNA3.1(+) Tag :
T2A-EGFP(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus DNA methyltransferase (cytosine-5) 1, fused with the EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL50066-2aP