Plasmid name :
pcDNA3.1(+)-MLKL(T357A/S358A)-Flag Gene/insert name :
MLKL(T357A/S358A) Alt name :
hMLKL Insert size :
1416bp Species :
Homo sapiens (human) Gene ID :
197259 Accessions :
NM_152649.3 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Nhe I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
EPI400 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
Mammalian Expression vector of Homo sapiens mixed lineage kinase domain like pseudokinase(two points mutation T357A/S358A) with C terminal Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-MLKL(T357A/S358A)-Flag Gene/insert name :
MLKL(T357A/S358A) Alt name :
hMLKL Insert size :
1416bp Species :
Homo sapiens (human) Gene ID :
197259 Accessions :
NM_152649.3 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Nhe I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
EPI400 Growth temperature :
37°C High or low copy :
Low Copy Plasmid description :
Mammalian Expression vector of Homo sapiens mixed lineage kinase domain like pseudokinase(two points mutation T357A/S358A) with C terminal Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL01031-2bP