Plasmid name :
pPLK/GFP+Puro-rMmp1 shRNA (3+1) Gene/insert name :
Mmp1 shRNA Alt name :
Clgn; MMP-1; Mmp1a Insert size :
65bp Species :
Rattus norvegicus (Norway rat) Gene ID :
300339 Accessions :
NM_001134530.1 Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pPLK/GFP+Puro-rMmp1 shRNA (3+1) Gene/insert name :
Mmp1 shRNA Alt name :
Clgn; MMP-1; Mmp1a Insert size :
65bp Species :
Rattus norvegicus (Norway rat) Gene ID :
300339 Accessions :
NM_001134530.1 Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8102bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL70029-3P