Plasmid name :
pcDNA3.1(+)-NOS3-3*Flag Gene/insert name :
NOS3 Alt name :
eNOS; ECNOS Insert size :
3612bp Species :
Homo sapiens (human) Gene ID :
4846 Accessions :
NM_000603 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens nitric oxide synthase 3(one point mutation T774C not causing the amino acid variation ) with C terminal 3*Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-NOS3-3*Flag Gene/insert name :
NOS3 Alt name :
eNOS; ECNOS Insert size :
3612bp Species :
Homo sapiens (human) Gene ID :
4846 Accessions :
NM_000603 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens nitric oxide synthase 3(one point mutation T774C not causing the amino acid variation ) with C terminal 3*Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL01876-2cP