Plasmid name :
pEnter-NT5E(T376A/M379T)-Flag-His Gene/insert name :
NT5E(T376A/M379T) Alt name :
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA Insert size :
1725 bp Species :
Homo sapiens (human) Gene ID :
4907 Accessions :
NM_002526 Vector Backbone :
pEnter Tag :
Flag(C-term);His(C-term) Backbone manufacturer :
Vigene Bioscience Vector type :
Mammalian Expression Backbone size w/o insert :
7510bp Cloning site 5' :
Not I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens 5'-nucleotidase ecto(two points mutation T376A/M379T) with C terminal Flag and 6xHis tag Selectable markers :
Puromycin
pEnter-NT5E(T376A/M379T)-Flag-His Gene/insert name :
NT5E(T376A/M379T) Alt name :
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA Insert size :
1725 bp Species :
Homo sapiens (human) Gene ID :
4907 Accessions :
NM_002526 Vector Backbone :
pEnter Tag :
Flag(C-term);His(C-term) Backbone manufacturer :
Vigene Bioscience Vector type :
Mammalian Expression Backbone size w/o insert :
7510bp Cloning site 5' :
Not I Site destroyed during cloning :
NO Cloning site 3' :
Xho I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
DH5alpha Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens 5'-nucleotidase ecto(two points mutation T376A/M379T) with C terminal Flag and 6xHis tag Selectable markers :
Puromycin
Blocking peptide available as PPL00480-2aP