Plasmid name :
pRK5-HA-PRAS40 Gene/insert name :
PRAS40 Alt name :
AKT1S1;Lobe;MGC2865;PRAS40 Insert size :
771bp Species :
Homo sapiens (human) Gene ID :
84335 Accessions :
BC007416.2 Vector Backbone :
pRK5-HA Tag :
2*HA(N-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
N/A Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT1 substrate 1 with N terminal 2xHA tag Selectable markers :
Neomycin
pRK5-HA-PRAS40 Gene/insert name :
PRAS40 Alt name :
AKT1S1;Lobe;MGC2865;PRAS40 Insert size :
771bp Species :
Homo sapiens (human) Gene ID :
84335 Accessions :
BC007416.2 Vector Backbone :
pRK5-HA Tag :
2*HA(N-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
N/A Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens AKT1 substrate 1 with N terminal 2xHA tag Selectable markers :
Neomycin
Blocking peptide available as PPL00180-2aP