Plasmid name :
pLKO.1-SHH shRNA-2 Gene/insert name :
SHH shRNA-2 Alt name :
TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5 Insert size :
53bp Species :
Homo sapiens (human) Gene ID :
6469 Accessions :
NM000193.2 Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian shRNA Knockdown vector of Homo sapiens sonic hedgehog Selectable markers :
Puromycin
pLKO.1-SHH shRNA-2 Gene/insert name :
SHH shRNA-2 Alt name :
TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5 Insert size :
53bp Species :
Homo sapiens (human) Gene ID :
6469 Accessions :
NM000193.2 Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian shRNA Knockdown vector of Homo sapiens sonic hedgehog Selectable markers :
Puromycin
Blocking peptide available as PPL00147-3eP