Plasmid name :
pLenti-CMV-GFP-Puro-Flag-Spp1(D93G/V139A/S159G/N205S/E234K) Gene/insert name :
Spp1 Alt name :
2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;OP; Opn; Opnl; Ric; Spp-1 Insert size :
888bp Species :
Mus musculus (house mouse) Gene ID :
20750 Accessions :
NM_00120420 Vector Backbone :
pLenti-CMV-GFP-Puro Tag :
Flag(N-term);CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
Xba I Site destroyed during cloning :
NO Cloning site 3' :
Not I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Mus musculus secreted phosphoprotein 1(five points mutation D93G/V139A/S159G/N205S/E234K) with the Flag tag at the N-terminus and unfused CopGFP protein at the C-terminus Selectable markers :
Puromycin
pLenti-CMV-GFP-Puro-Flag-Spp1(D93G/V139A/S159G/N205S/E234K) Gene/insert name :
Spp1 Alt name :
2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;OP; Opn; Opnl; Ric; Spp-1 Insert size :
888bp Species :
Mus musculus (house mouse) Gene ID :
20750 Accessions :
NM_00120420 Vector Backbone :
pLenti-CMV-GFP-Puro Tag :
Flag(N-term);CopGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression, Lentiviral Backbone size w/o insert :
8189bp Cloning site 5' :
Xba I Site destroyed during cloning :
NO Cloning site 3' :
Not I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Lentiviral expression vector of Mus musculus secreted phosphoprotein 1(five points mutation D93G/V139A/S159G/N205S/E234K) with the Flag tag at the N-terminus and unfused CopGFP protein at the C-terminus Selectable markers :
Puromycin
Blocking peptide available as PPL50113-4aP