Plasmid name :
pIRES2-EGFP-3*Flag-Tmbim1 Gene/insert name :
Tmbim1 Alt name :
2310061B02Rik; AA960455; AU024746; C78899;mKIAA4161; RECS1; Tmbib1 Insert size :
930bp Species :
Mus musculus (house mouse) Gene ID :
69660 Accessions :
NM_027154 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus transmembrane BAX inhibitor motif containing 1 with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-Tmbim1 Gene/insert name :
Tmbim1 Alt name :
2310061B02Rik; AA960455; AU024746; C78899;mKIAA4161; RECS1; Tmbib1 Insert size :
930bp Species :
Mus musculus (house mouse) Gene ID :
69660 Accessions :
NM_027154 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
EcoR I Site destroyed during cloning :
NO Cloning site 3' :
BamH I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Mus musculus transmembrane BAX inhibitor motif containing 1 with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL50128-2aP