Plasmid name :
pIRES2-EGFP-3*Flag-hWWP2 Gene/insert name :
WWP2 Alt name :
AIP2; WWp2-like Insert size :
2613bp Species :
Homo sapiens (human) Gene ID :
11060 Accessions :
NM_007014.4 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens WW domain containing E3 ubiquitin protein ligase 2(Identical with the NCBI sequence except for three point mutations G1239A/T1707C/A2091G not causing the amino acid variation) with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-3*Flag-hWWP2 Gene/insert name :
WWP2 Alt name :
AIP2; WWp2-like Insert size :
2613bp Species :
Homo sapiens (human) Gene ID :
11060 Accessions :
NM_007014.4 Vector Backbone :
pIRES2-EGFP Tag :
3*Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens WW domain containing E3 ubiquitin protein ligase 2(Identical with the NCBI sequence except for three point mutations G1239A/T1707C/A2091G not causing the amino acid variation) with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL01781-2aP